TABLE 3

Cycle thresholds for real-time PCR amplification using TaqMan primers specific for KATP channel subunits in whole-rat mesenteric LVs

Transcripts encoding all four known KATP channel subunits were detected, including the pore-forming Kir6.1 and Kir6.2 subunits and the regulatory SUR1 and SUR2 subunits. However, the SUR1 transcript was extremely sparse. GAPDH was the amplification standard. Data reported as mean ± S.D., n = 3.

Channel SubunitsCycle Thresholds
Kir6.125.9 ± 1.6
Kir6.224.0 ± 1.6
SUR131.6 ± 1.3
SUR225.3 ± 1.7
GAPDH25.7 ± 3.0