TABLE 2

Antagonist effects in cellular functional assay (human receptors)

For assay details, see https://www.eurofinsdiscoveryservices.com/cms/cms-content/services/in-vitro-assays/.

AssaySourceMeasured ComponentStimulusEmbedded ImageEmbedded Image
α2ACHO cellsBioimpedanceAdrenaline(100 nM)35 μM10 μM
α2CCHO cellsFluorescence(HTRF)Adrenaline(1 μM)n.c.an.c.a
5-HT7CHO cellscAMPSerotonin(300 nM)∼1000 μM
5-HT2AHEK-293 cellsIP1Serotonin(100 nM)150 μM21 μM
5-HT2CHEK-293 cellsIP1Serotonin(10 nM)3700 μM830 μM
  • a n.c.; Embedded Image not calculable. CHO, chinese hamster ovary; HEK, human embryonic kidney; HTRF, homogenous time-resolved fluorescence; IP, inositol phosphate.