Isobolographic analysis showing theoretical (EF50 add) and experimentally determined (EF50 mix) values of GX and MDZ combinations against the 6-Hz-induced seizures
| AED Combination | Fixed Ratio | GX + MDZ = ED50 mix (mg/kg) | nmix | EF50 add = GX + MDZ (mg/kg) | nadd | ||||
|---|---|---|---|---|---|---|---|---|---|
| GX + MDZ | 1:3 | 0.834 | 0.076 | 0.910 (0.608–0.364) | 32 | 0.665 (0.268–1.237) | 0.365 | 0.3 | 32 |
| GX + MDZ | 1:1 | 0.432 | 0.118 | 0.550 (0.368–0.823) | 32 | 0.93 (0.426–1.784) | 0.73 | 0.2 | 32 |
| GX + MDZ | 3:1 | 0.223 | 0.181 | 0.404 (0.259–0.631) | 32 | 1.195 (0.583–2.330) | 1.095 | 0.1 | 32 |
nadd, total cell numbers used to calculate the theoretical line of additivity; nmix, total cell numbers in each mixture of drug combinations.