Isobolographic analysis showing theoretical (EF50 add) and experimentally determined (EF50 mix) values of GX and MDZ combinations against the 6-Hz-induced seizures

AED CombinationFixed RatioGX + MDZ = ED50 mix (mg/kg)nmixEF50 add = GX + MDZ (mg/kg)nadd
GX + MDZ1:30.8340.0760.910 (0.608–0.364)320.665 (0.268–1.237)0.3650.332
GX + MDZ1:10.4320.1180.550 (0.368–0.823)320.93 (0.426–1.784)0.730.232
GX + MDZ3:10.2230.1810.404 (0.259–0.631)321.195 (0.583–2.330)1.0950.132
  • nadd, total cell numbers used to calculate the theoretical line of additivity; nmix, total cell numbers in each mixture of drug combinations.