TABLE 3

Cellular IC50 values for loss of spheroid viability after 48-hour exposure

Exponentially growing human breast and ovarian cancer cells were plated in ultralow attachment U-bottom microtiter plates and cultured for 24 hours to allow spheroid formation. Compounds were added to the preexisting spheroids and microtiter plates were incubated for 48 hours. Cell viability was determined with CellTiterGlo. N = 3, mean ± S.E.M.

Compound (μM) ± S.E.M.
MDA-MB-231Hs578TOVCAR4Kuramochi
JMS-05332.67 ± 7.028.48 ± 2.384.42 ± 1.0413.25 ± 0.65
EJR-866-75>50 μM12.01 ± 3.8419.64 ± 4.66a>50 μM
EJR-866-81>50 μM14.39 ± 4.9112.35 ± 2.26a>50 μM
NRT-870-5961.54 ± 9.66a10.07 ± 1.9311.50 ± 2.38a34.14 ± 8.60a
JMS-038>50 μM>50 μM>50 μM>50 μM
  • a P < 0.05 compared with JMS-053.