TABLE 1

[³H]DHTB binding in subcellular fractions

Data represent mean ± S.E.M. for each treatment group from three independent experiments, using pooled tissue from 12–15 mice per genotype per treatment group.

Fraction	Treatment	Genotype	B_max ± S.E.M.	K_d ± S.E.M.
			pmol/mg protein	nM
Synaptosomal	Saline	Taar1 WT	1.4 ± 0.2	2.3 ± 0.5
		Taar1 KO	1.5 ± 0.2	2.4 ± 0.4
	MA	Taar1 WT	1.3 ± 0.2	2.3 ± 0.4
		Taar1 KO	1.3 ± 0.2	2.3 ± 0.2
Membrane-associated	Saline	Taar1 WT	1.9 ± 0.3	2.3 ± 0.5
Membrane-associated		Taar1 KO	2.0 ± 0.5	2.3 ± 0.7
	MA	Taar1 WT	1.9 ± 0.3	2.3 ± 0.6
		Taar1 KO	1.5 ± 0.3	2.4 ± 0.5
Vesicular	Saline	Taar1 WT	1.0 ± 0.2	2.2 ± 0.3
		Taar1 KO	1.2 ± 0.1	3.4 ± 1.0
	MA	Taar1 WT	0.8 ± 0.2*	1.2 ± 0.1*
		Taar1 KO	0.6 ± 0.1*	1.1 ± 0.1*

^* P < 0.05 compared with saline treatment (main effect).