TABLE 1

CNP-38 conjugates used for in vivo and in vitro studies

CNP-38 Conjugate (Linker Type, Linker Attachment Site)Description
5-kDa PEG-linker CNP-38 (permanent, Lys26)Linear 5-kDa PEG carrier conjugated to the Lys26 side chain of CNP-38 via a permanent linker
5-kDa PEG-linker CNP-38 (permanent, N-terminus)Linear 5-kDa PEG carrier conjugated to the N-terminus of CNP-38 via a permanent linker
4 × 10-kDa PEG-linker CNP-38 (permanent, Lys26)4-Arm–branched 4 × 10-kDa PEG carrier conjugated to the Lys26 side chain of CNP-38 via a permanent linkera
2 × 20-kDa PEG-linker CNP-38 (transient, Lys26)2-Arm–branched 2 × 20-kDa PEG carrier conjugated to the Lys26 side chain of CNP-38 via a TransCon linkera
4 × 10-kDa PEG-linker CNP-38 (transient, Lys26), TransCon CNP4-Arm–branched 4 × 10-kDa PEG carrier conjugated to the Lys26 side chain of CNP-38 via a TransCon linkera
Cb(10-kDa PEG-linker CNP-38)4 (permanent, Lys26)Four CNP-38 molecules conjugated to a 4-arm–branched 4 × 10-kDa PEG; one CNP-38 is attached via its Lys26 side chain and via a permanent linker to the distal end of each of the four PEG arms

  • ↵a The linker attachment site to the PEG carrier is close to the branching site of the PEG arms.

  • ↵b C represents the central carbon atom, which is the branching point of the four PEG arms.