TABLE 1

Comparison of different biodistribution studies of T cells in mice. Methods of T cell isolation, expansion, and labeling, as well as tissue biodistribution at 20-24 hours are compared.

MethodWallace et al. (1993)Melder et al. (2002)Present Study
LabelingI-125 PKH95–labeled T cellsIn-111 oxine–labeled T cellsCr-51–labeled T cells
Preparation of T cellsSplenocytes exposed to 6000 IU/ml IL-2TILs extracted from MC38 s.c. tumorsLymphocytes extracted from spleenMice sensitized with splenocytes of MCaIV tumor-bearing C3H mice and lymphocytes extracted from spleenSplenocytes expanded using anti-CD3, anti-CD28, and IL-2TILs extracted from B16-BL6 s.c. tumors
Tissues listed in descending order of concentrations at 20–24 hSpleenLungSpleenLungSpleenLung
LungSpleenLiverSpleenLiverSpleen
LiverLiverLungLiverLungLiver
SkinSkinTumorTumorKidneyKidney
MuscleMuscleHeartHeartTDLNTumor