TABLE 2

Primers and PCR conditions used for CYP1A2 genotyping

Single Nucleotide Polymorphism (SNP)	Primer Name	Sequence 5′→ 3′	PCR Program	[Mg²⁺]	PCR Product Size	Restriction Enzyme	Incubation Temperature	Genotype Patterns
				mM	bp		°C
rs2069514 (−3860 G→A)	Forward 3, reverse 2 (Fw3Rv2)	GCTACACATGATCGAGCTATAC	(94°C 10 min) and 35× (94°C 30 s, 56°C 30 s, 72°C 1 min)	2.0	568	BslI	55	G/G:343-132-93
rs2069514 (−3860 G→A)	Forward 3, reverse 2 (Fw3Rv2)	CAGGTCTCTTCACTGTAAAGTTA	(94°C 10 min) and 35× (94°C 30 s, 56°C 30 s, 72°C 1 min)	2.0	568	BslI	55	A/A:475-93
rs762551 (−163 C→A)	Forward 1, reverse 1 (Fw1Rv1)	TGAGGCTCCTTTCCAGCTCTCA	(94°C 10 min) and 35× (94°C 30 s, 60°C 30 s, 72°C 1 min)	2.5	265	PspOMI	37	C/C:211-54
rs762551 (−163 C→A)	Forward 1, reverse 1 (Fw1Rv1)	AGAAGCTCTGTGGCCGAGAAGG	(94°C 10 min) and 35× (94°C 30 s, 60°C 30 s, 72°C 1 min)	2.5	265	PspOMI	37	A/A:265