TABLE 3

Effect of vehicle, TAS-303, or duloxetine on NE-induced contraction of rat isolated urethral strips

EC50 of NE-induced contractions was obtained from a sigmoidal curve under variable maximum effect conditions. Results are shown as mean ± S.E.M. (n = 5 for each compound in each tissue). P > 0.05 vs. control (pretreatment).

CompoundConcentrationEC50P Value
nMlog10 mol/l
Vehicle−4.49 ± 0.09
First vehicle−4.54 ± 0.11N.S.
Second vehicle−4.57 ± 0.08N.S.
Third vehicle−4.62 ± 0.07N.S.
Fourth vehicle−4.64 ± 0.04N.S.
Fifth vehicle−4.63 ± 0.08N.S.
Duloxetine−4.40 ± 0.08
10−4.43 ± 0.08*
30−4.57 ± 0.06**
100−4.80 ± 0.02**
300−5.00 ± 0.08**
1000−5.12 ± 0.04**
TAS-303−4.35 ± 0.10
10−4.48 ± 0.12N.S.
30−4.69 ± 0.08**
100−4.77 ± 0.08**
300−4.95 ± 0.08**
1000−5.12 ± 0.11**
  • * P < 0.05; **P < 0.01 vs. control (pretreatment) by a paired Student’s t test.