Comet assay of DDI treatment in combination with FF-10502 or gemcitabine in human pancreatic SUIT-2 cells under serum starvation
Percentage of fluorescence intensity in tail DNA region per whole DNA is expressed as % tail DNA. The experiment was performed in duplicate, and the mean % tail DNA was calculated.
| Group | % Tail DNA | |||||||
|---|---|---|---|---|---|---|---|---|
| No DDIs | 5 µmol/l Cisplatin | 300 µmol/l H2O2 | 200 µmol/l TMZ | |||||
| 4 h | 24 h | 4 h | 24 h | 4 h | 24 h | 4 h | 24 h | |
| Vehicle control (0.1% DMSO) | 5.5 | 7.4 | 12.1 | 11.3 | 38.9 | 6.1 | 40.3 | 39.5 |
| FF-10502 100 nmol/l | 8.9 | 17.1 | 20.1 | 45.1 | 83.1 | 89.7 | 71.8 | 93.7 |
| FF-10502 1000 nmol/l | 6.8 | 22.2 | 19.9 | 49.7 | 93.8 | 95.8 | 88.1 | 97.4 |
| Gemcitabine 100 nmol/l | 5.6 | 6.5 | 19.5 | 13.1 | 37.4 | 6.9 | 39.0 | 39.5 |
| Gemcitabine 1000 nmol/l | 5.2 | 6.8 | 13.4 | 15.9 | 43.2 | 7.5 | 48.0 | 38.6 |
TMZ, temozolomide.