Target gene and primer sequence
| Target Gene | RT-PCR Primer Sequence (5′-3′) |
|---|---|
| C4ST-1 | Forward 5′ AGG GTC GTG CAG TTT CCT TA 3′ |
| Reverse 5′ AGG GTT AGG GGG TTT CTG GT 3′ | |
| ChSy-1 | Forward 5′-GAA GAG GAC GGG GAC AGC-3′ |
| Reverse 5′-CCT TTA AGA GGG GAC CAT GC-3′ | |
| XT-1 | Forward 5′-AAG GTC CGC ATA CCA AAC AC-3′ |
| Reverse 5′-TAT CCT CAT GGG GTG GT-3′ | |
| Biglycan | Forward 5′-CTC AAC TAC CTG CGC ATC TCA G-3′ |
| Reverse 5′-GAT GGC CTG GAT TTT GTT GTG-3′ |