TABLE 1

Primers used in this study for mutagenesis, cloning, and RT-qPCR (5′–3′)

PrimerSequence (5′-3′)
Site directed mutagenesis
 miR331-5p_FGATAGCATTTGGAGATCGCAACAATGTTAAATGACGA
 miR331-5p_RTCGTCATTTAACATTGTTGCGATCTCCAAATGCTATC
 miR-331-5p/delMT_FGGGGAAGGATAGCATTTGGAGATATGTTAAATGACGAGT
 miR-331-5p/delMT_RACTCGTCATTTAACATATCTCCAAATGCTATCCTTCCCC
 MT B_F and MT C_FGACAAAATTTATTTTAGCGAGATTTAATACGTAC
 MT B_R and MT C_RGTACGTATTAAATCTCGCTAAAATAAATTTTGTC
 MT D_F and MT E_FTTTTCCAGGGATTTACGCAGTACTTTAGCTGGAA
 MT D_R and MT E_RTTCCAGCTAAAGTACTGCGTAAATCCCTGGAAAA
 MT1_FGAGGATTTCCTTTTAGACTTGACAAAACATCTTTTC
 MT1_RGAAAAGATGTTTTGTCAAGTCTAAAAGGAAATCCTC
 MT2_FCATCTTTTCACAACTGTAGCTGTTAAGACAAAATT
 MT2_RAATTTTGTCTTAACAGCTACAGTTGTGAAAAGATG
 MT3_FGACAAAATTTATTTTAGCTAGATCTAGAGTCGGGGC
 MT3_RGCCCCGACTCTAGATCTCGCTAAAATAAATTTTGTC
 MT4_FCCTTGTTAAGACAAACGAGCTTTTCCAGGGATCTAG
 MT4_RCTAGATCCCTGGAAAAGCTCGTTTGTCTTAACAAGG
 MT5_FTCCTTTTTCCTGTGAAGCGCCATCTTTTCACAAC
 MT5_RGTTGTGAAAAGATGGCGCTTCACAGGAAAAAGGA
 MT6_FTGTGACAAAACATCTCAGAGCAACTTACCTTGTTAAG
 MT6_RCTTAACAAGGTAAGTTGCTCTGAGATGTTTTGTCACA
Cloning
 2B15/UTR_F1CCGCTCTAGATTATATCAAAAGCCTGAAGT
 2B15/UTR (1–75)_RCCGCTCTAGACATTTAAAACCCTCCATGCT
 2B15/UTR (1–150)_RCCGCTCTAGATCCCTGGAAAATAAATTTTG
 2B15/UTR (1–225)_RCCGCTCTAGAATCCTTCCCCCCATTGTATT
 2B15/UTR (1–314)_RCCGCTCTAGAGCAGGTTAGCTACATATGTA
 2B15/UTR (1–384)_RCCGCTCTAGAAAGAGTTGTATTTTTTTTTT
 3UTR_F2CCGCTCTAGAAATGTTAAATGACGAGTTAC
 2B15/UTR (244–318)_RCCGCTCTAGAGCAGGTTAGCTACATATGTA
 2B15/UTR (244–384)_RCCGCTCTAGAAAGAGTTGTATTTTTTTTTT
 2B15/UTR (244–454)_RCCGGTCTAGAGACACTTTATTTTCAGATCC
RT-qPCR
 miR331-5p_qPCR_FGCAGCTAGGTATGGTCCCA
 miR-331-5p_qPC_RCCAGTTTTTTTTTTTTTTTGGATCCC
  • RT-qPCR, reverse-transcriptase quantitative real-time polymerase chain reaction.