PCR primers used to determine the expression of relevant genes in 231-CON and 231-TXL cells
| Gene | PCR Forward Primers (5′-3′) | PCR Reverse Primers (5′-3′) |
|---|---|---|
| ABCB1 | AGGCCAACATACATGCCTTC | CCTTCTCTGGCTTTGTCCAG |
| ABCC1 | AGGTGGACCTGTTTCGTGAC | TCCACCAGAAGGTGATCCTC |
| ABCG2 | TTATCCGTGGTGTGTCTGGA | TTCCTGAGGCCAATAAGGTG |
| TOP1 | AGTCCGGCATGATAACAAGG | GCCGAGCAGTCTCGTATTTC |
| TOP2A | TGGCTGAAGTTTTGCCTTCT | GGCCTTCTAGTTCCACACCA |
| TOP2B | GAGTGGCTTGTGGGAATGTT | TGTGCTTCTTTCCAGGCTTT |
| GAPDH | GAGTCAACGGATTTGGTCGT | TTGATTTTGGAGGGATCTCG |
| β-actin | GGACTTCGAGCAAGAGATGG | AGCACTGTGTTGGCGTACAG |
| PPIA | ACCGCCGAGGAAAACCGTGT | CTGTCTTTGGGACCTTGTCTGCA |