TABLE 1

Primers used for cloning, site-directed mutagenesis, and RT-qPCR

PrimerSequence (5′–3′)
Cloning
 UGT2B7_3′-UTR_FCCGCTCTAGATTATATCTGAGATTTGAAGC
 UGT2B7_3′-UTR_RCCGGTCTAGACCGTAGTGTTTTCTTCATTG
 UGT2B4_3′-UTR_FCCGGTCTAGATTACGTCTGAGGCTGGAAGC
 UGT2B4_3′-UTR_RCCGATCTAGAGCTTCCTCAACAACAGTTAA
Site-directed mutagenesis
 2B7_miR3664_MT_FAGATTTCTTTCTTAGCTAGACAAAAAAAAAAAAAG
 2B7_miR3664_MT_RTTTTTTTTTTTTGTCTAGCTAAGAAAGAAATCTTG
 2B4_miR135a_MT_FCAAAAATGATATAAATGACTATGAGGTTATATTG
 2B4_miR135a_MT_RCAATATAACCTCATAGTCATTTATATCATTTTTG
 2B4_miR410_MT_FAAAGCCATATGAGGTCCGCTTGAAATGTATTGAG
 2B4_miR410_MT_RCTCAATACATTTCAAGCGGACCTCATATGGCTTT
RT-qPCR
 miR3664-3p_qPCR_FGCAGTCTCAGGAGTAAAGACA
 miR3664-3p_qPCR_RAGGTCCAGTTTTTTTTTTTTTTTAACTC
 miR-1266-5p_qPCR_FGCC TCA GGG CTG TAG AAC A
 miR-1266-5p_qPCR_RGTC CAG TTT TTT TTT TTT TTT AGC CC
 miR-4483_qPCR_FGCA GGG GGT GGT CTG T
 miR-4483_qPCR_RGGT CCA GTT TTT TTT TTT TTT TCA ACA
 miR-4317_qPCR_FGCA GAC ATT GCC AGG GA
 miR-4317_qPCR_RCAG GTC CAG TTT TTT TTT TTT TTT AAA C
 miR-216b-5p_qPCR_FGCA GAA ATC TCT GCA GGC A
 miR-216b-5p_qPCR_RGGT CCA GTT TTT TTT TTT TTT TTC ACA TT
 miR-135a_qPCR_FCGC AGT ATG GCT TTT TAT TCC T
 miR-135a_qPCR_RGGT CCA GTT TTT TTT TTT TTT TTC ACA T
 miR-410_qPCR_FCGC AGA ATA TAA CAC AGA TGG C
 miR-410_qPCR_RAGG TCC AGT TTT TTT TTT TTT TTA CAG
 miR-489_qPCR_FCGC AGG TGA CAT CAC ATA TAC
 miR-489_qPCR_RCCA GTT TTT TTT TTT TTT TGC TGC C
 miR-4691-5p_qPCR_FGGT CCT CCA GGC CAT GA
 miR-4691-5p_qPCR_RAGT TTT TTT TTT TTT TTC CGC AGC
 miR-101_qPCR_FCGC AGT ACA GTA CTG TGA TA
 miR-101_qPCR_RGGT CCA GTT TTT TTT TTT TTT TTT CAG T
  • F, forward; R, reverse.