TABLE 2 

Expression of Myo/Nog and muscle cell markers in 30-day human lens cultures

Anterior human lens explants were cultured for 30 days and doubled-labeled with antibodies to G8 and noggin, MyoD, α-SMA, and sarcomeric myosin and fluorescent secondary antibodies. The results are the mean ± standard deviation. The number of subject is indicated in parentheses.

MarkersPercent Positive
G86 ± 4 (18)
Noggin5 ± 3 (7)
MyoD1 ± 2 (4)
Myosin4 ± 3 (4)
α-SMA9 ± 5 (9)
G8+ cells with Noggin100 (4)
Noggin+ cells with G899 ± 3 (4)
G8+ cells with MyoD24 ± 24 (4)
MyoD+ cells with G8100 (4)
G8+ with myosin53 ± 29 (4)
Myosin+ cells with G8100 (4)
G8+ cells with α-SMA 82 ± 22 (6)
α-SMA+ cells with G867 ± 32 (6)
  • % positive = (number of fluorescent cells ÷ total number of cells in 20 fields) × 100. % G8+ with second primary antibody = (number of G8+ cells co-labeled with the second primary antibody ÷ total G8+ cells) × 100. Reciprocal pairs are also presented. All G8+ cells contained Noggin. Subpopulations of Myo/Nog cells contain different muscle proteins.