Expression of Myo/Nog and muscle cell markers in 30-day human lens cultures
Anterior human lens explants were cultured for 30 days and doubled-labeled with antibodies to G8 and noggin, MyoD, α-SMA, and sarcomeric myosin and fluorescent secondary antibodies. The results are the mean ± standard deviation. The number of subject is indicated in parentheses.
| Markers | Percent Positive |
|---|---|
| G8 | 6 ± 4 (18) |
| Noggin | 5 ± 3 (7) |
| MyoD | 1 ± 2 (4) |
| Myosin | 4 ± 3 (4) |
| α-SMA | 9 ± 5 (9) |
| G8+ cells with Noggin | 100 (4) |
| Noggin+ cells with G8 | 99 ± 3 (4) |
| G8+ cells with MyoD | 24 ± 24 (4) |
| MyoD+ cells with G8 | 100 (4) |
| G8+ with myosin | 53 ± 29 (4) |
| Myosin+ cells with G8 | 100 (4) |
| G8+ cells with α-SMA | 82 ± 22 (6) |
| α-SMA+ cells with G8 | 67 ± 32 (6) |
% positive = (number of fluorescent cells ÷ total number of cells in 20 fields) × 100. % G8+ with second primary antibody = (number of G8+ cells co-labeled with the second primary antibody ÷ total G8+ cells) × 100. Reciprocal pairs are also presented. All G8+ cells contained Noggin. Subpopulations of Myo/Nog cells contain different muscle proteins.