TABLE 1 

Depletion of Myo/Nog cells with G8:3DNA:Dox in human lens cultures

Anterior human lens explants were incubated with medium alone (untreated), G8:3DNA, 3DNA:Dox, or G8:3DNA:Dox for 24 hours. Cells were double-labeled with the G8 mAb and fluorescent secondary antibodies, and TUNEL reagents. The results are the mean ± standard deviation. Untreated, G8:3DNA and 3DNA:Dox, n = 5; G8:3DNA:Dox: n = 8. Significant differences were found between the percentages of TUNEL+ cells in all three control groups compared with G8:3DNA:Dox (P < 0.0001), untreated and 3DNA:Dox (P = 0.0005), and G8:3DNA and 3DNA:Dox (P = 0.02). % G8+ or TUNEL+ = (number of fluorescent cells ÷ total number of cells in 20 fields) × 100. % G8+ with TUNEL = (number of G8+ cells colabeled with the second primary antibody ÷ total G8+ cells) × 100. Reciprocal pairs are also presented. G8:3DNA:Dox, but not control conjugates, specifically killed G8+ cells.

Treatment% G8+% TUNEL+% G8+ with TUNEL% TUNEL+ with G8
Untreated4 ± 10.7 ± 0.200
G8:3DNA4 ± 10.5 ± 0.300
3DNA:Dox4 ± 20.1 ± 0.100
G8:3DNA:Dox5 ± 15 ± 199 ± 299 ± 2