List of PCR primers used for UGT2B 3′ UTR PCR amplification, cloning, and luciferase mutational analysis
| Primer | Sequence |
|---|---|
| Outer UGT2B7 3′ UTR For | 5′ – CCTTCGGGTTGCAGCCCACGAC – 3′ |
| Outer UGT2B7 3′ UTR Rev | 5′ – ATAGGCTCTCAGGATTCAGAGGGGAGGG – 3′ |
| Inner UGT2B7 3′ UTR For | 5′ – GCTATCTAGAGTTATATCTGAGATTTGAAGCTGGAAAACC – 3′ |
| Inner UGT2B7 3′ UTR Rev | 5′ – GCTATCTAGATAAGGCTTTATCTTATTTTTTATTTTCCG – 3′ |
| UGT2B4 3′ UTR For | 5′ – GCTATCTAGATTACGTCTGAGGCTGGAAGCTG – 3′ |
| UGT2B4 3′ UTR Rev | 5′ – GCTATCTAGACACAATCCTGCATGAAATGATCC – 3′ |
| UGT2B10 3′ UTR For | 5′ – GCTATCTAGAGGGATTAGTTATATCTGAGATTTGAAGCTGG – 3′ |
| UGT2B10 3′ UTR Rev | 5′ – GCTATCTAGACCTAAGTCATCATGACCATGGCTCAGAGTG – 3′ |
| UGT2B7 216b Del. SDM For | 5′ – CCTTGTCAAATAAAAATTTGTTTTTCAGTTACCACCCAGTTCATGGTT – 3′ |
| UGT2B7 216b Del. SDM Rev | 5′ – TAACCATGAACTGGGTGGTAACTGAAAAACAAATTTTTATTTGACAAAG – 3′ |
| UGT2B4 216b Del. SDM For | 5′ – TTATTACAACAATAAGACGTTGTGATACAATTCCTTTCTTCTTGTG – 3′ |
| UGT2B4 216b Del. SDM Rev | 5′ – CACAAGAAGAAAGGAATTGTATCACAACGTCTTCTTGTTGTAATAA – 3′ |
| UGT2B10 216b Del. SDM For | 5′ – CTACCTTGTCAAGTAAAATTTGTTTTTCATTTACCACCCAGTTAATG – 3′ |
| UGT2B10 216b Del. SDM Rev | 5′ – CCATTAACTGGGTGGTAAATGAAAAACAAATTTTTACTTGACAAGGTAG – 3′ |
| UGT2B10 SNP rs139538767 SDM For | 5′ – CAAGTAAAAATTTGTTTTTCAGAGGTTTACCACCCAGTTAATGGTTAG – 3′ |
| UGT2B10 SNP rs139538767 SDM Rev | 5′ – CTAACCATTAACTGGGTGGTAAACCTCTGAAAAACAAATTTTTACTTG – 3′ |
| miR-216b-5p | 5′ – AAATCTCTGCAGGCAAATGTGA – 3′ |
Del, deletion; For, forward; Rev, reverse. Underlined sequences refer to the XbaI enzyme recognition site within the primer.