TABLE 1

CM-157 binding parameters, in the absence and presence of TH-chrysenediol, calculated by different fitting protocols

Condition	One Site		Two Sites				Dimer
Condition	K_D	B_max	K_DHigh	K_DLow	B_High	B_Low	K₁	K₂	R_T^{^a}
	nM	RU	nM		RU		nM		RU
CM-157	19	2634	1.8	18	517	2330	7.5	86.7	1460
CM-157 + 10 nM TH-chrysenediol	61	2570	76.0	66.09	1176	1458	30.4	121	1284
CM-157 + 100 nM TH-chrysenediol	67	837	>9000	89	—	910	46.3	61	375

RU, relative unit.
↵^a R_T is the total amount of dimers; it should be roughly equivalent to B_max/2. Unlike in radioligand binding studies, the B_max parameter does not indicate the actual level of binding sites, but it may be used for comparative purposes.