CM-157 binding parameters, in the absence and presence of TH-chrysenediol, calculated by different fitting protocols

ConditionOne SiteTwo SitesDimer
CM-1571926341.8 18517 23307.5 86.71460
CM-157 + 10 nM TH-chrysenediol61257076.0 66.091176 145830.4 1211284
CM-157 + 100 nM TH-chrysenediol67837>9000 8991046.3 61375
  • RU, relative unit.

  • a RT is the total amount of dimers; it should be roughly equivalent to Bmax/2. Unlike in radioligand binding studies, the Bmax parameter does not indicate the actual level of binding sites, but it may be used for comparative purposes.