Relative neutral endopeptidase (NEP) inhibition in tissues after single dose oral treatments of normal rats
| Race 1 h | Candox 1 h | Sac 1 h | UK 1 h | Race 5 h | Candox 5 h | Sac 5 h | UK 5 h | |
|---|---|---|---|---|---|---|---|---|
| Kidney | 56% | 99% | 100% | 99% | 39% | 45% | 90% | 86% |
| Lung | 75% | 98% | 99% | 99% | 59% | 52% | 79% | 94% |
| Duodenum | 98% | 100% | 100% | 100% | 85% | 97% | 99% | 99% |
| Jejunum | 90% | 100% | 99% | 100% | 32% | 92% | 97% | 98% |
NEP inhibitors were administered to normal healthy rats by oral gavage at the following doses: racecadotril (Race) 100 mg/kg, candoxatril (Candox) 150 mg/kg, sacubitril (Sac) 100 mg/kg, and UK-505,749 (UK) 100 mg/kg. Tissue lysates were prepared after 1 and 5 h, and NEP activity was measured as described in Materials and Methods. Percentage inhibition is the relative reduction in activity compared with vehicle-treated control animals.