TABLE 1

Inhibition of 3H-glyburide transport in BCRP-overexpressing cells and placental BeWo cells

Transport IC50a
HEK WT-BCRPHEK C421A-BCRPBeWo
Ko143 (nM)21.5 ± 1.8110.1 ± 3.11b3.62 ± 3.39
Genistein (µM)4.65 ± 0.273.92 ± 1.260.18 ± 0.22

  • ↵a IC50 value calculated using Graphpad nonlinear regression analysis (dose-response: [log] inhibitor versus response. Variable slope (four parameters)) and presented as mean ± S.D. (n = 3 or 4 independent experiments)

  • ↵b P < 0.05 compared with WT-BCRP control.