TABLE 2

Compounds 9 and 16 increase affinity and mazindol decreases affinity of the hDAT for [125I]RTI-55

None of the treatment conditions had a significant effect on the Bmax values. All Bmax P values are >0.05; one-way analysis of variance; Dunnett’s multiple comparisons test; n = 3–12.

Treatment
CompoundConcentrationKi (95% Confidence Interval)Bmax ± S.E.M.
nMfmol/mg protein
Control1.31 (0.92–1.89)7040 ± 590
910 nM0.902 (0.58–1.39)7820 ± 1170
930 nM0.60 (0.29–1.23)*6960 ± 520
9100 nM0.39 (0.15–1.01)**7280 ± 540
9300 nM0.53 (0.42–0.67)*7730 ± 880
Control1.20 (0.96–1.50)6460 ± 420
1630 nM1.09 (0.50–2.38)7240 ± 1560
16100 nM0.94 (0.51–1.72)6700 ± 410
16300 nM0.60 (0.38–0.95)*7390 ± 770
161 µM0.58 (0.33–1.00)***7140 ± 580
Control1.24 (0.99–1.54)6510 ± 350
Mazindol10 nM2.30 (1.66–3.18)*6310 ± 1150
Mazindol30 nM2.77 (0.67–1.14)***6360 ± 480
  • * P < 0.05, **P < 0.001, *** P < 0.01.