Chemicals used and their functions and concentrations
| Chemical | Function | Concentration | Reference |
|---|---|---|---|
| Acetylcholinesterase | Enzyme responsible for acetylcholine degradation | 4 U/ml | Pohanka (2011) |
| AMTB | TRP channel subfamily M member 8 (TRPM8) inhibitor | 10−6 M | Lashinger et al. (2008) |
| Atropine | Muscarinic receptor inhibitor | 10−5 M | Clark (1926) |
| Bromoacetylcholine | Choline acetyltransferase inhibitor | 10−6 M | Tucek (1982) |
| HC-030031 | TRP channel, subfamily A, member 1 (TRPA1) inhibitor | 10−6 M | Earley et al. (2009) |
| HC-067047 | TRP cation channel subfamily V member 4 (TRPV4) inhibitor | 10−6 M | Vincent and Duncton (2012) |
| Hemicholinium-3 | High-affinity choline transporter inhibitor | 10−7 M | Hartmann et al. (2008) |
| L-NAME | NO synthase inhibitor | 10−5 M | Rees et al. (1990) |
| Mecamylamine | Nicotinic receptor inhibitor | 10−4 M | Bacher et al. (2009); Zou et al. (2012) |
| TRAM-34 | Intermediate-conductance calcium-activated potassium channels inhibitor | 5 × 10−7 M | Gluais et al. (2005) |
| UCL 1684 | Small-conductance calcium-activated potassium channels inhibitor | 5 × 10−7 M | Gluais et al. (2005) |
| Vesamicol | VAChT inhibitor | 10−6 M | Prior et al. (1992) |