Kinetics of binding to human or cynomolgus PCSK9 by surface plasmon resonance at 25°C, pH 7.4
Data are mean ± S.D., for n replicates.
| Form of Adnectin | KD | Kon | Koff |
|---|---|---|---|
| nM | 1/Ms | 1/second | |
| Human PCSK9 | |||
| 1459D05 (progenitor) | 1.58 ± 0.18 (n = 3) | 1.13E+05 | 1.80E-4 |
| BMS-962476, his6, no PEG | 0.29 ± 0.01 (n = 2) | 2.42E+05 | 7.03E-05 |
| BMS-962476 his6, PEGylated | 0.65 | 1.23E+05 | 8.03E-05 |
| BMS-962476 | 0.85 ± 0.3 (n = 3) | 6.86E+04 | 5.61E-05 |
| Cynomolgus PCSK9 | |||
| BMS-962476, his6, no PEG | 8.1 | 2.49E+05 | 1.77E-03 |
| BMS-962476 | 18.2 | 2.25E+05 | 4.11E-03 |