TABLE 2

Primer pairs for real-time polymerase chain reaction

Forward and reverse primers for each gene are listed. Common gene symbols are shown and, where appropriate, official HUGO gene symbols are given in brackets. Generic primers were used for genes that can encode multiple isoforms.

GeneOligonucleotideAccession Number(s)
p57kip2 (CDKN1C)NM_000076.2, NM_001122630.1, NM_001122631.1
     Forward5′-CTG TCC GGG CCT CTG ATC T-3′
     Reverse5′-CAT CGC CCG ACG ACT TCT-3′
CRISPLD2NM_031476.3
     Forward5′-CAA ACC TTC CAG CTC ATT CAT G-3′
     Reverse5′-GGT CGT GTA GCA GTC CAA ATC C-3′
GILZ (TSC22D3)NM_198057.2, NM_004089.3, NM_001015881.1
     Forward5′-TGG CCA TAG ACA ACA AGA TCG A-3′
     Reverse5′-CAC AGC ATA CAT CAG ATG ATT CTT CA-3′
SOCS3NM_003955.3
     Forward5′-GAT TCT CCT TCA ATT CCT CAG CTT-3′
     Reverse5′-ATT AGT TCA GCA TTC CCG AAG TGT-3′
CD200NM_005944.5, NM_001004196.2
     Forward5′-GGA CTG TGA CCG ACT TTA AGC AA-3′
     Reverse5′-AGC AAT AGC GGA ACT GAA AAC C-3′
TTP (ZFP36)NM_003407.2
     Forward5′-GGA CTG TGA CCG ACT TTA AGC AA-3′
     Reverse5′-AGC AAT AGC GGA ACT GAA AAC C-3′
BRF-1 (ZFP36-L1)NM_004926.3, NM_001244698.1, NM_001244701.1
     Forward5′-TCG ACT TGA GCG AAG TTT TAT GC-3′
     Reverse5′-CCC TGC ACT GGG AGC ACT AT-3′
RGS2NM_002923
     Forward5′-CCT CAA AAG CAA GGA AAA TAT ATA CTG A-3′
     Reverse5′-AGT TGT AAA GCA GCC ACT TGT AGC T-3′
GAPDHNM_002046.4, NM_001256799.1
     Forward5′-ATG GAA ATC CCA TCA CCA TCT T-3′
     Reverse5′-CAG CAT CGC CCC ACT TG-3′-