List of primers used for real-time polymerase chain reaction analysis
| Gene Name | Primer | Sequence (5′ to 3′) | Location |
|---|---|---|---|
| Rat NFATc1 | c1-p1 | 5′-CCC AGC GGT CGG CCC AGG AAC T-3′ | 1779–1800 |
| (XM_001058445) | c1-p2 | 5′-GCG TAG GCG GGA GAG GAA AGG TCG TG-3′ | 2346–2321 |
| c1-p3 | 5′-CAG CTG CCG TCG CAC TCT GGT C-3′ | 1268–1289 | |
| c1-p4 | 5′-CCC GGC TGC CTT CCG TCT CAT A-3′ | 1361–1340 | |
| Rat NFATc2 | c2-p1 | 5′-CAC GGC CGG CTC TGC TGT TCT CA-3′ | 953–975 |
| (XM_001072834) | c2-p2 | 5′-TGG TGG CCC GCA TGT TGT TCT TTG-3′ | 1602–1579 |
| c2-p3 | 5′-TGG GGC CGT GTG AGC AGG AGG AGA G-3′ | 1120–1144 | |
| c2-p4 | 5′-AGT CAC GGG GAT GCT GCA GAT GGG AAT G-3′ | 1238–1211 | |
| Rat NFATc3 | c3-p1 | 5′-GGA ACA GCC GAT GAT AGA TAT TTA CG AC-3′ | 1707–1734 |
| (XM_001074460) | c3-p2 | 5′-GAC CCC CTT GAC ATT TTT CTC TGA TTA T-3′ | 2235–2208 |
| c3-p3 | 5′-CCC CAT CCC GGG AGA CAT CAG TAG-3′ | 1357–1380 | |
| c3-p4 | 5′-TAT AGG AGT GTG GCC AGG CTT TGG TTT G-3′ | 1493–1466 | |
| Rat NFATc4 | c4-p1 | 5′-CAG GGC GGC GGG AAG GTC GTC-3′ | 1918–1938 |
| (NM_001107264) | c4-p2 | 5′-AGG AAA GGG GCG CGG AAG AAA GGA GA-3′ | 2604–2579 |
| c4-p3 | 5′-GGG AGG AAG AGG CCG CAG TGA AC-3′ | 2129–2151 | |
| c4-p4 | 5′-CCG CCC ATT GGA GAC ATA AAA GTA GAC C-3′ | 2256–2229 | |
| Rat CCR2 | CCR2-p1 | 5′-AGG GGG CCA CCA CAC CGT ATG A-3′ | 92–113 |
| (NM_021866) | CCR2-p2 | 5′-CAT GTT GCC CAC AAA ACC AAA GAT GAA-3′ | 222–196 |
| Rat S18 | S18-p1 | 5′-ACT TTT GGG GCC TTC GTG TC-3′ | 419–438 |
| (NM_213557) | S18-p2 | 5′-GCC CAA AGA CTC ATT TCT TCT TG-3′ | 514–492 |