TABLE 1

Kinetic analysis of the effect of UGT2A1_i2 coexpression on UGT2A1_i1 activity against PAH substrates

Data are expressed as milligram of total protein homogenate, corrected for relative UGT2A1_i1 expression. KM and Vmax represent the mean of three independent experiments.

1-OH-pyrene3-OH B[a]PB[a]P-7,8-diol
KMVmaxVmax/KMKMVmaxVmax/KMKMVmaxVmax/KM
μMpmol/min/mgμl/min/mgμMpmol/min/mgμl/min/mgμMpmol/min/mgμl/min/mg
Vehicle control159 ± 16319 ± 12*2.0 ± 0.08*256 ± 3282 ± 4.3*0.32 ± 0.04*230 ± 2286 ± 3.6*0.37 ± 0.04*
2 μM PonA140 ± 16261 ± 111.9 ± 0.09276 ± 2074 ± 2.20.27 ± 0.02253 ± 3770 ± 4.60.28 ± 0.05
6 μM PonA145 ± 17219 ± 8.81.5 ± 0.1261 ± 2464 ± 2.40.24 ± 0.005224 ± 1953 ± 2.00.24 ± 0.02
10 μM PonA138 ± 12153 ± 5.61.1 ± 0.08238 ± 2447 ± 1.90.20 ± 0.01205 ± 3241 ± 2.60.20 ± 0.01
  • * p(trend) < 0.01.