AUC (μmol · h/l) of concentration of ATA and AUC of total concentration of 2-OH-ATA and 4-OH-ATA without and with coincubation of E3S in fresh human hepatocytes, cryopreserved human hepatocytes, and HepaRG cells in suspension at day 0
The significance levels of difference in the average loss of ATA and formation of ATA hydroxy metabolites with and without coincubation of E3S are shown. Results shown are average ± S.D.
| Cells | Experiment | AUC ATA | AUC OH-ATA |
|---|---|---|---|
| Fresh hepatocytes | ATA | 152 ± 3 | 64 ± 20 |
| Fresh hepatocytes | ATA + E3S (30 μM) | 191 ± 13* | 51 ± 23* |
| Cryo hepatocytes | ATA | 164 ± 7 | 67 ± 17 |
| Cryo hepatocytes | ATA + E3S (30 μM) | 196 ± 23* | 47 ± 11* |
| HepaRG | ATA | 206 ± 20 | 52 ± 7 |
| HepaRG | ATA + E3S (30 μM) | 207 ± 21 | 50 ± 6 |
↵* , P < 0.05.