TABLE 1

Effects of FCS on the activation of 2×GRE-dependent transcription and the enhancement by LABAs

BEAS-2B cells harboring a 2×GRE reporter were not treated (naive) or were treated with formoterol (10 nM) (Form) or salmeterol (100 nM) (Salm) in the presence of either no stimulation (NS) or dexamethasone (1 μM) (Dex). Cells were pretreated for 1 h, or not, with 10% FCS. After 6 h, cells were harvested for luciferase assay. Data are from Fig. 6 plus additional experiments and are expressed as fold activation ± S.E. relative to untreated. In addition, enhancement by LABAs was calculated relative to naive or dexamethasone treatment.

NSDexFCSFCS + Dex
NActivationNActivationEnhancement by LABANActivationNActivationEnhancement by LABA
Naive141.0147.1 ± 0.31141.2 ± 0.1143.8 ± 0.3§§§1
Form41.0 ± 0.0921.1 ± 1.4***3.0 ± 0.240.9 ± 0.396.5 ± 1.1*,§§§2.2 ± 0.5#
Salm40.9 ± 0.0921.5 ± 0.9***3.0 ± 0.140.8 ± 0.397.0 ± 1.0**,§§§2.0 ± 0.1###
  • ANOVA with a Dunnett's multiple comparison to compare the activation by Dex with activation by Dex plus Form or Salm (in both the absence and the presence of FCS): * P < 0.05; * P < 0.01; *** P < 0.001. Paired t test to examine the effects of FCS on the enhancement by Dex, Dex + Form, and Dex + Salm: §§§ P < 0.001. Paired t test (one-tailed) between the enhancement by LABA in the absence and presence of FCS: # P < 0.05; ### P < 0.001.