The forward mutagenic primers used for preparation of the polymorphic mutants of CYP2B6
The base pairs that contain the desired mutations are underlined. The single mutants were prepared using the plasmid of CYP2B6*1 as templates, whereas the double and triple mutants were prepared using the plasmids of CYP2B6*4 and CYP2B6*6 as templates, respectively, as described under Materials and Methods.
CYP2B6 | Amino Acid | Mutagenic Primers |
---|---|---|
CYP2B6*4 | K262R | GACCCCAGCGCCCCCCGCGACCTCATCGACACC |
CYP2B6*5 | R487C | CAACATACCAGATCTGCTTCCTGCCCCGC |
CYP2B6*6 | Q172H/K262R | CYP2B6*4 + GACCCCACCTTCCTCTTCCATTCCATTACCGC |
CYP2B6*7 | Q172H/K262R/R487C | CYP2B6*6 + CAACATACCAGATCTGCTTCCTGCCCCGC |
CYP2B6*8 | K139E | AGGGACTTCGGGATGGGAGAACGGAGTGTGGAG |
CYP2B6*9 | Q172H | GACCCCACCTTCCTCTTCCATTCCATTACCGC |