Potency, efficacy, and persistence of action of β-AR agonists in CHO cells recombinantly expressing human β2-AR and β1-AR
The average of three independent experiments performed in triplicate is shown. The rightward shift (EC50 shift) was calculated by dividing the washed EC50 by the unwashed EC50.
| Agonist | Human β2-AR CHO Cells | Human β1-AR CHO Cells | ||||
|---|---|---|---|---|---|---|
| pEC50 Control | pEC50 Washed | EC50 Shift | pEC50 Control | pEC50 Washed | EC50 Shift | |
| Isoprenaline | 8.88 ± 0.04 | 6.15 ± 0.07 | 538 | 9.55 ± 0.10 | 6.66 ± 0.05 | 759 |
| Fenoterol | 8.37 ± 0.06 | 5.66 ± 0.08 | 513 | N.T. | N.T. | N.T. |
| Salbutamol | 7.15 ± 0.11 | 5.11 ± 0.15 | 1202 | N.T. | N.T. | N.T. |
| Formoterol | 9.83 ± 0.08 | 7.81 ± 0.05 | 105 | N.T. | N.T. | N.T. |
| Salmeterol | 10.11 ± 0.07 | 9.39 ± 0.10 | 5 | 6.88 ± 0.03 | 5.73 ± 0.05 | 14 |
| Olodaterol | 10.00 ± 0.05 | 9.1 ± 0.04 | 8 | 7.98 ± 0.05 | 6.03 ± 0.04 | 89 |
N.T., not tested.