TABLE 2

Met336 substitutions with amino acids with different physical–chemical properties significantly alter the sensitivity to ethanol and IVM

Data of 50 mM ethanol are adopted from Popova et al. (2010). Data are presented as mean ± S.E.M. of 3 to 10 oocytes per data point. Significant differences were found for all the mutants except for the ethanol effect in Phe mutant (P < 0.05 compared with the WT P2X4R).

IVM, 3 μMEtOH, 50 mMATP EC10 Range
% Potentiation% InhibitionμM
Met (WT)208 ± 2422.0 ± 1.61.0–1.2
Ala1154 ± 519.3 ± 4.60.5–0.8
Val737 ± 426.5 ± 3.70.8–1.0
Phe324 ± 2519.0 ± 3.50.8–1.0
Trp531 ± 965.9 ± 2.50.25–0.3
Gln508 ± 774.0 ± 5.50.6–0.8
Glu15.5 ± 5.9−1.0 ± 3.60.6–0.8
Arg−4.8 ± 1.80.8 ± 1.40.1–0.2