TABLE 3

Derived kinetic parameters for codeine glucuronidation by recombinant UGT2B4 and UGT2B7 determined in the absence and presence of 2% (w/v) bovine serum albumin

Data are ented as mean ± standard error of parameter fit. Kinetic constants were derived from fitting with the Hill equation.

Without BSAWith 2% BSA
S50VmaxnCLmaxaS50VmaxnCLmaxa
mMpmol/min·mgμl/min·mgmMpmol/min·mgμl/min·mg
UGT2B42.61 ± 0.00179 ± 0.021.19 ± 0.0010.020.32 ± 0.00437 ± 0.161.50 ± 0.030.06
UGT2B71.07 ± 0.0349 ± 0.631.17 ± 0.020.030.27 ± 0.0153 ± 0.441.22 ± 0.030.12
  • a CLmax calculated as Vmax/S50× (n −1)/n(n − 1)1/n.