TABLE 2

Derived kinetic parameters for codeine glucuronidation by human liver microsomes determined in the absence and presence of 2% (w/v) bovine serum albumin

	Without BSA^{^a},^{^b}			With 2% BSA^{^a},^{^c}
	K_m	V_max	CL_int^{^d}	K_m	V_max	K_si	CL_int^{^d}
	mM	pmol/min·mg	μl/min·mg	mM	pmol/min·mg	mM	μl/min·mg
H7	2.35 ± 0.01	440 ± 1.2	0.19	0.32 ± 0.01	430 ± 9.7	8.20 ± 1.04	1.34
H10	2.68 ± 0.02	312 ± 0.9	0.12	0.34 ± 0.01	303 ± 6.2	9.49 ± 1.19	0.89
H12	3.13 ± 0.18	920 ± 20.8	0.29	0.28 ± 0.02	848 ± 23.8	14.42 ± 2.78	3.03
H13	2.29 ± 0.12	754 ± 13.2	0.33	0.26 ± 0.001	680 ± 0.7	10.63 ± 0.12	2.62
H40	1.16 ± 0.01	438 ± 0.5	0.38	0.24 ± 0.01	447 ± 6.6	14.46 ± 1.50	1.86
Mean ± S.D.	2.32 ± 0.73	573 ± 253	0.26 ± 0.11	0.29 ± 0.04	541 ± 218	11.44 ± 2.87	1.95 ± 0.88
Pooled HLM	2.15 ± 0.13	584 ± 16	0.27	0.23 ± 0.01	436 ± 12	8.15 ± 1.03	1.90

↵a Data presented as mean ± standard error of parameter fit.
↵b Kinetic constants derived from fitting with the Michaelis Menten equation.
↵c Kinetic constants derived from fitting with the substrate inhibition equation.
↵d CL_int calculated as V_max/K_m for both Michaelis-Menten and substrate inhibition kinetics.