TABLE 1

Summary of binding assay conditions for rat receptors

Receptor	Tissue^{^a}	Incubation Buffer	Incubation Time, Temperature, pH	Radioligand^{^b}	Nonspecific^{^c}	Reference
rD₃	Sf9 (8)	50 mM Tris-HCl, 10 mM MgCl₂, 1 mM EDTA, 120 mM NaCl	60 min, 27°C, 7.4	[³H]Spiperone (0.85)	Haloperidol (10)	Sokoloff et al. (1990)
rD₂	Striatum (250)	50 mM Tris-HCl, 1 mM MgCl₂, 5 mM KCl, 2 mM CaCl₂, 120 mM NaCl, 0.1% ascorbic acid	15 min, 37°C, 7.4	[³H]Spiperone (0.5)	(+)Butaclamol (10)	Creese et al. (1979)
r5-HT_1A	Hippocampus (450)	50 mM Tris-HCl, 4 mM CaCl₂, 0.01 mM pargyline, 0.1% ascorbic acid	15 min, 37°C, 7.7	[³H]8-OH-DPAT (2.0)	Serotonin (10)	Hall et al. (1985)
r5-HT_2A	Cerebral cortex (170)	50 mM Tris-HCl	15 min, 37°C, 7.4	[³H]Ketanserin (1.1)	Methysergide (1)	Gozlan et al. (1986)