Table 1

Generation of hCAR chimeric constructs

Vector Names	Mutagenesis Primers	Source Vector	Introduced Amino Acids
hCAR1+YLT	`5′-CATGGCCCTCTTCTCTCCTTATCTTACAGACCGACCTG-3′`	pCR3/hCAR1	YLT (271–273)
	`5′-CAGGTCGGTCTGTAAGATAAGGAGAGAAGAGGGCCATG-3′`		YLT (271–273)
hCAR1+AP	`5′-CTCTTCTCTCCTGCTCCCGACCGACCTGGAGTTACC-3′`	pCR3/hCAR1	AP (271–272)
	`5′-GGTAACTCCAGGTCGGTCGGGAGCAGGAGAGAAGAG-3′`		AP (271–272)
hCAR1+A	`5′-CCCTCTTCTCTCCTGCTGACCGACCTGGAGTTAC-3′`	pCR3/hCAR1	A (271)
	`5′-GTAACTCCAGGTCGGTCAGCAGGAGAGAAGAGGG-3′`		A (271)
hCAR1+P	`5′-CCTCTTCTCTCCTCCCGACCGACCTGGAG-3′`	pCR3/hCAR1	P (271)
	`5′-CTCCAGGTCGGTCGGGAGGAGAGAAGAGG-3′`		P (271)