Apparent permeability coefficients (Papp × 10–6 cm/s) and net efflux ratio of bidirectional [3H]digoxin and [14C]mannitol transport for 60 min across the Caco-2 cell monolayer Caco-2 cells were treated with 1,25(OH)2D3 (10 and 100 nM) on day 18 for three consecutive days. The transport experiment was performed on day 21 for 60 min. Results are mean ± S.D. in triplicates.
Treatment | Papp for [3H]Digoxina | Papp for [14C]Mannitolb | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| A→B | B→A | EfR | A→B | B→A | EfR | |||||
| Control PBS | 1.7 ± 0.4 | 11.5 ± 1.8 | 6.8 | 0.7 ± 0.2 | 1.5 ± 0.4 | 2.2 | ||||
| Ethanol (0.1%) | 2.1 ± 0.4 | 11.8 ± 0.6 | 5.8 | 0.8 ± 0.3 | 1.1 ± 0.1 | 1.3 | ||||
| 1,25(OH)2D3 (10 nM) | 2.1 ± 0.3 | 12.7 ± 1.4 | 6.1 | 1.2 ± 0.2 | 1.7 ± 0.2 | 1.4 | ||||
| 1,25(OH)2D3 (100 nM) | 1.9 ± 0.3 | 15.1 ± 0.5* | 8.0 | 1.3 ± 0.3 | 1.9 ± 0.7 | 1.5 | ||||