Primer sets for quantitative real-time PCR
Gene | GenBank No. | Forward (5′→3′ Sequence) | Reverse (5′→3′ Sequence) |
|---|---|---|---|
| GAPDHa | NM_002046 | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC |
| VDR | NM_001017535 | GACATCGGCATGATGAAGGAG | GCGTCCAGCAGTATGGCAA |
| CYP3A1 | NG_008421 | CATTCCTCATCCCAATTCTTGAAG T | CCACTCGGTGCTTTTGTGTATCT |
| CYP1A2 | NM_000761 | CAAGGGACACAACGCTGA | CCAGGACTTCCCCGATACA |
| SULT1E1 | NM_005420 | AAACAATTAGATGAGATGAAT | ATTTGGATGACCAGCCAC |
| ASBT | NM_000452 | GCCCCAAAAAGCAAAGATCA | GCTATGAGCACAATGAGGATGG |
| PEPT1 | NM_005073 | TCCACCGCCATCTACCATAC | GGACAAACACAATCAGGGCT |
| MDR1 | NM_000927 | TGCTCAGACAGGATGTGAGTTG | AATTACAGCAAGCCTGGAACC |
| MRP2 | NM_000392 | CAAACTCTATCTTGCTAAGCAGG | TGAGTACAAGGGCCAGCTCTA |
| MRP3 | NM_003786 | CTTAAGACTTCCCCTCAACATGC | GGTCAAGTTCCTCTTGGCTC |
| MRP4 | NM_005845 | AATGTGACCGTCCATCCTCC | AGGTTTGGCCTTCTTGGGA |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase