TABLE 5

Effect of SoRI-9804 on d-amphetamine-induced [3H]MPP+ efflux [3H]MPP+ efflux experiments were conducted as described under Materials and Methods to determine the effect of SoRI-9804 on d-amphetamine-induced (100 nM) [3H]MPP+ efflux. The data of three independent experiments were pooled and fit to one- and two-component dissociation experiments using MLAB-PC. The parameter values are ± S.D. In all cases, the two-component model fit significantly better than the one-component model (F-test, P < 0.001).


Condition

K1

A1

K2

A2
min–1 % min–1 %
d-Amph (100 nM) 0.027 ± 0.01 22 ± 6 0.19 ± 0.02 88 ± 5
d-Amph (100 nM) + SoRI-9804 (0.4 μM) 0.028 ± 0.013 33 ± 18 0.14 ± 0.04 74 ± 16
d-Amph (100 nM) + SoRI-9804 (2.0 μM) 0.035 ± 0.01 59 ± 12* 0.21 ± 0.07 50 ± 10*
d-Amph (100 nM) + SoRI-9804 (10 μM) 0.021 ± 0.004 58 ± 11* 0.15 ± 0.05 45 ± 10*
d-Amph (100 nM) + SoRI-9804 (25 μM)
0.023 ± 0.004
65 ± 10*
0.19 ± 0.06
43 ± 9*
  • * The A values of the curve (constraint 4; see Materials and Methods) were significantly different (F-test, P < 0.01) from the 100 nM d-Amph curve. The kinetic constants of each curve did not significantly differ from those of the 100 nM d-Amph curve

  • The entire curve (constraint 1; see Materials and Methods) was significantly different (F-test, P < 0.01) from the 100 nM d-Amph curve