TABLE 2

Effect of test agents on substrate-induced [3H]MPP+ release Eight point dose-response curves were generated using [3H]MPP+ in the absence and presence of the indicated test agents. The data of three independent experiments were pooled (30 data points) and fit to the dose-response equation for the best-fit estimate of the EC50 and Emax (± S.D.) values.


Drug

EC50 [Ke]

Emax
nM %
Experiment 1 (Fig. 6A)
   Dopamine 57 ± 5 95 ± 2
   + SoRI-20040 (10 μ M) 158 ± 12* 64 ± 1*
   + SoRI-9804 (10 μ M) 124 ± 9* 70 ± 1*
   + (–)-cocaine (2.8 μM) 608 ± 40* [0.29 μM] 92 ± 1
Experiment 2 (Fig. 6B)
   MDA 110 ± 10 104 ± 2
   + SoRI-20040 (10 μ M) 265 ± 39* 68 ± 2*
   + SoRI-9804 (10 μ M) 195 ± 12* 74 ± 1*
   + (–)-cocaine (2.8 μM)
1744 ± 225* [0.19 μM]
106 ± 3
  • * P < 0.05 compared with the value of the control condition (ANOVA with a post hoc Bonferroni test)