TABLE 4

Kinetic parameters of 3TC uptake in CHO-hOCT1, -hOCT2, and - hOCT3 The Km and Vmax values were estimated by fitting data of Fig. 6 to a Michaelis-Menten nonlinear equation. Values are mean ± S.E.M. of at least three experiments.




Km

Vmax

Vmax/Km
mM nmol/mg protein/min μl/mg protein/min
hOCT1 1.25 ± 0.10 10.04 ± 0.32 8.03 ± 0.40
hOCT2 1.90 ± 0.25 7.80 ± 0.45 4.10 ± 0.30
hOCT3
2.14 ± 0.24
9.27 ± 0.50
4.30 ± 0.31