TABLE 2

Kd for high-affinity binding site and low-affinity binding site and p values for statistical comparison of two-binding versus one-binding site fitting of inhibition curve shown in Fig. 2 Data are shown as Kd values ± S.E.M. from at least three independent experiments with each point performed in quadruplicate.




High-Affinity Binding Site

Low-Affinity Binding Site

Two-Binding vs. One-Binding Site
pM mM
hOCT1 12.30 ± 0.25 1.90 ± 0.14 p<0.0001
hOCT2 8.13 ± 0.17 3.45 ± 0.11 p<0.0001
hOCT3
15.40 ± 0.23
2.40 ± 0.13
p<0.0001