TABLE 2

Physiological parameters determined in control and fructose-treated rats 2 weeks after injection of empty pcDNA3.1 vector or pcDNA3.1-eNOS N + pcDNA3.1, N + pcDNA-eNOS, and normal groups were given control water, and F + pcDNA3.1 and F + pcDNA-eNOS groups were given water containing 10% fructose. N + pcDNA3.1 and F + pcDNA3.1 groups were injected with empty pcDNA3.1 vector, N + pcDNA-eNOS and F + pcDNA-eNOS groups were injected with pcDNA3.1-eNOS, and the normal group was injected with saline. All parameters were measured at week 4 of the study (i.e., after 4 weeks on the respective water treatments and 2 weeks after injections).


Variable

N + pcDNA3.1 (n = 6)

N + pcDNA-eNOS (n = 6)

Normal (n = 6)

F + pcDNA3.1 (n = 10)

F + pcDNA-eNOS (n = 10)
Glucose (mM) 4.59 ± 0.25 4.78 ± 0.47 4.31 ± 0.33 4.81 ± 0.61 4.62 ± 0.63
Triglyceride (mM) 0.49 ± 0.11 0.44 ± 0.14 0.48 ± 0.13 0.58 ± 0.22 0.57 ± 0.13
Cholesterol (mM) 1.38 ± 0.09 1.42 ± 0.11 1.41 ± 0.12 1.44 ± 0.24 1.40 ± 0.21
Insulin (mIU/l) 9.43 ± 0.78 9.55 ± 1.09 9.40 ± 0.95 13.10 ± 0.95* 9.25 ± 1.12#
HOMA-IR 1.87 ± 0.12 1.78 ± 0.15 1.82 ± 0.13 2.88 ± 0.29* 1.88 ± 0.18#
Body weight (g) 250 ± 11 254 ± 9 249 ± 16 250 ± 7 246 ± 12
Urine volume (ml/day/100 g) 4.5 ± 0.6 4.2 ± 0.8 4.2 ± 0.4 20.9 ± 3.6* 14.8 ± 4.0
Urine osmolarity (mOsml/kg H2O) 834 ± 109 1,381 ± 157* 903 ± 32 169 ± 18* 387 ± 77#
Water consumption volume (ml/day/100 g) 5.95 ± 1.63 4.78 ± 0.51 5.40 ± 2.98 40.1 ± 1.74* 34.29 ± 2.16*#
Urine sodium (mmol/24 h)
719.8 ± 241.7
666.9 ± 120.3
700.3 ± 169
1475.1 ± 329.8*
1150 ± 337.9
  • * P < 0.05 vs. N + pcDNA3.1 group

  • # P < 0.05 vs. F + pcDNA3.1 group