TABLE 1

Influence of various inhibitors on the effect of N-arachidonoyl l -serine in HEK293 hSlo cells Cells were incubated with solvents (control) or with 14,15-EEZE, staurosporine, indomethacin, NDGA, URB597, 1-aminobenzotriazole (for 30 min), or pertussis toxin (12 h) or were infused with GDP-β-S for 5 min before experiment. The current was generated before (Itime0) and 30 s after the application of 3 μM ARA-S or vehicle (Iligand) by a voltage step of 98.3 ± 0.88 mV (control for GDP-β-S) and 114.5 ± 1.71 mV (GDP-β-S), corresponding to 0.2 Gmax or by ∼90 mV (other treatment groups). Results are presented as means ± S.E.M. (n = number of experiments).

Treatment
Itime0
Iligand/Itime0
n
Vehicle
ARA-S
nA
Control 2.34 ± 0.13 1.00 ± 0.01 1.57 ± 0.02* 6
GDP-β-S, 2 mM 2.27 ± 0.13 0.98 ± 0.03 1.55 ± 0.04* 6
Control 1.71 ± 0.09 1.00 ± 0.01 1.51 ± 0.03* 8
Pertussis toxin, 1 μg/ml 1.87 ± 0.18 1.00 ± 0.01 1.49 ± 0.02* 12
Control 1.49 ± 0.09 1.02 ± 0.02 1.59 ± 0.07* 5
14,15-EEZE, 20 μM 1.60 ± 0.07 1.01 ± 0.01 1.43 ± 0.05* 5
Control 1.81 ± 0.17 0.96 ± 0.06 1.68 ± 0.03* 6
Staurosporine, 1 μM 1.84 ± 0.15 0.99 ± 0.11 1.65 ± 0.05* 6
Control 1.67 ± 0.20 0.93 ± 0.05 1.80 ± 0.02* 3
Indomethacin, 10 μM 1.70 ± 0.63 0.94 ± 0.01 1.81 ± 0.01* 3
Control 1.48 ± 0.12 0.97 ± 0.01 1.73 ± 0.03* 4
NDGA, 10 μM 1.62 ± 0.11 0.97 ± 0.01 1.82 ± 0.01* 3
Control 1.59 ± 0.11 0.97 ± 0.03 1.73 ± 0.02* 3
URB597, 10 μM 1.64 ± 1.36 0.96 ± 0.06 1.57 ± 0.07* 3
Control 2.07 ± 0.11 0.97 ± 0.08 1.73 ± 0.03* 4
1-Aminobenzotriazole, 20 μM
2.09 ± 0.21
1.00 ± 0.10
1.63 ± 0.05*
5

  • ↵* P < 0.001 compared with vehicle