TABLE 2

Ligand potencies at hC5a and hC5L2 receptor as measured by radioligand binding with [¹²⁵I]hC5a

Ligand	Recombinantly Expressed Receptor^*
Ligand		hC5aR	hC5L2R
hC5a^**	0.05 ± 0.01 nM	0.85 ± 0.07 nM
hC5a desArg^**	14.1 ± 1.7 nM	16.2 ± 3.0 nM
NDT 9513727	11.6 ± 5.3 nM	>10 μM

↵* The CHO cell expression system was used for all examples except NDT 9513727 activity at the hC5aR, which used baculovirus-expressed C5aR. All values shown are IC₅₀ values
↵** Synthesized and purified using His-tag methodologies (see Materials and Methods)