TABLE 2

Transport rate and permeability-surface area product of methotrexate across the intestinal everted sac prepared from wild-type and Abcc3–/– mice Data were taken from Fig. 4. The transport rate was obtained by multiplying the concentration in the perfusate at 40 to 45 min by the flow rate. The permeability-surface area product for the net methotrexate permeation from the mucosal side to the serosal side (PSnet) was calculated by dividing the transport rate at 40 to 45 min by the concentration in the mucosal medium. Each value represents the mean ± S.D. (duodenum: n = 6 or 7; jejunum: n = 7; ileum: n = 4; colon: n = 3 or 4).



Concentration

Transport Rate

PSnet

Wild-Type
Abcc3–/–
Wild-Type
Abcc3–/–
μM nmol/min/sac μl/min/sac
Duodenum 22 0.0717 ± 0.0448 0.0306 ± 0.0081* 3.26 ± 2.04 1.39 ± 0.37*
Duodenum 2200 1.45 ± 0.59 1.26 ± 0.42 0.660 ± 0.269 0.573 ± 0.190
Jejunum 22 0.00963 ± 0.00568 0.0105 ± 0.0057 0.438 ± 0.258 0.479 ± 0.261
Ileum 22 0.00367 ± 0.00203 0.00397 ± 0.00216 0.167 ± 0.092 0.181 ± 0.098
Colon
22
0.00499 ± 0.00368
0.00632 ± 0.00236
0.227 ± 0.167
0.287 ± 0.107
  • * P < 0.05, significantly different from wild-type mice