TABLE 1
pEC50 values for the maximal elevation of [Ca2+]i by NmU analogs in HEK 293 cells transiently expressing either rNmU-R1 or rNmU-R2
Using a FLIPR, concentration-response curves were generated for the maximal (peak) changes in fluorescence (as an index of changes in [Ca2+]i) in response to the various NmU analogs in populations of HEK 293 cells that had been transiently transfected with either rNmU-R1 or rNmU-R2. Data were fit to sigmoidal curves by a four-parameter logistic equation using GraphPad Prism. Data are mean ± S.E.M., n = 3.