TABLE 2

Peripheral inflammatory injury does not alter the number (Bmax) or affinity (Kd) of μ -opioid receptors in the rostral ventromedial medulla

Saturation isotherms were generated for the binding of [3H]DAMGO to membranes containing the rostral ventromedial medulla of rats 4 h, 4 days, or 2 weeks after intraplantar injection of saline or CFA in one hindpaw. Values are the mean ± S.E.M. of three to four independent experiments.


Intraplantar Treatment

[3H]DAMGO
Bmax
Kd
fmol/mg protein nM
4 h
    Saline 28.1 ± 1.9 0.9 ± 0.2
    CFA 26.6 ± 3.9 1.0 ± 0.2
4 Days
    Saline 26.7 ± 4.4 0.7 ± 0.1
    CFA 27.9 ± 2.9 0.7 ± 0.1
2 Weeks
    Saline 18.0 ± 0.9 0.7 ± 0.1
    CFA
20.7 ± 2.3
1.0 ± 0.3