TABLE 3

Hepatic microsomal CPR activity, P450 content, and rates of CPA 4-hydroxylation

Liver microsomes were prepared from individual 3-month-old male mice. CPR activity was determined using cytochrome c as the electron acceptor. P450 content was determined spectrally. Rates of 4-OH-CPA formation from CPA were determined as described under Materials and Methods. Reaction mixtures contained 0.2 or 2.0 mM CPA. The values presented are means ± S.E. (n = 4 mice/group). Values in parentheses are percentages. Data were log-transformed before statistical analysis using Tukey's test.

Strain	CPR Activity	P450 Content	Rate of 4-OH-CPA Formation
Strain	CPR Activity	P450 Content			0.2 mM CPA	2.0 mM CPA
	nmol cytochrome c reduced / min / mg protein	nmol / mg protein	nmol / min / mg protein
Wild type	155 ± 7 (100)	0.48 ± 0.05 (100)	0.60 ± 0.13	2.14 ± 0.32
LCN	4.0 ± 0.5^a (2.5)	2.48 ± 0.18^a (516)	0.12 ± 0.02^b	0.42 ± 0.06^b
CL	68 ± 6^a (45)	0.98 ± 0.14 (204)	0.77 ± 0.08	2.50 ± 0.17
CL-LCN	2.0 ± 0.2^a,^c (1.5)	2.95 ± 0.11^a (615)	0.10 ± 0.02^b	0.31 ± 0.03^b

↵ a P < 0.01, compared with wild type.
↵ b P < 0.05, compared with wild type.
↵ c P < 0.05, compared with LCN.