TABLE 5

Effect of albumin preparations on the inhibition of AZT glucuronidation by fatty acids with recombinant UGT2B7 as the enzyme source

Incubations were conducted with 400 μM AZT as substrate, ± albumin and ± fatty acids. Data represent mean of duplicate estimations. BSA is unmodified (or crude).


Albumin Preparation

18.1n-9 Conc.

Rate of GAZT Formation

18.2n-6 Conc.

Rate of GAZT Formation

20.4n-6 Conc.

Rate of GAZT Formation

FAMa Conc.

Rate of GAZT Formation
μM pmol / min · mg μM pmol / min · mg μM pmol / min · mg μM pmol / min · mg
None 0 19.6 0 19.6 0 19.6 0 19.6
3 15.0 3 13.3 1.5 8.1 7.5 10.4
BSA 0 38.3 0 38.3 0 38.3 0 38.3
3 37.4 3 38.3 1.5 36.8 7.5 38.7
HSA-FAF 0 31.3 0 31.3 0 31.3 0 31.3

3
31.3
3
32.4
1.5
31.2
7.5
31.6
  • a FAM, fatty acid mixture comprising oleic (18.1n-9), linoleic (18.2n-6), and arachidonic (20.4n-6) acids; individual concentrations were 3 μM 18.1n-9, 3 μM 18.2n-6, and 1.5 μM 20.4n-6.