TABLE 1

Effect of bovine serum albumin preparations on the kinetics of zidovudine glucuronidation by human liver microsomes and recombinant UGT2B7

Data for HLM are the mean ± S.D. of four replicates, whereas data for UGT2B7 represent the mean of duplicate experiments. Crude refers to unmodified BSA.

Enzyme Source
Parameter
No Albumin
Crude
FAF
GF
FAFGF
0.5%
2.0%
0.5%
2.0%
0.5%
2.0%
0.5%
2.0%
HLM Km, μM 1113 ± 56 105 ± 6* 87 ± 2* 124 ± 3* 125 ± 14* 211 ± 12* 188 ± 4* 135 ± 6* 136 ± 6*
Vmax, pmol/min · mg 738 ± 13 746 ± 27 867 ± 40 859 ± 6 785 ± 19 755 ± 34 715 ± 54 810 ± 44 900 ± 10
CLint, μl/min · mg 0.66 7.11 9.97 6.93 6.28 3.58 3.80 6.02 6.62
UGT2B7 Km, μM 439 75 40 75 44 95 97 102 99
Vmax, pmol/min · mg 50 43 56 48 56 47 48 45 46
CLint, μl/min · mg
0.113
0.569
1.408
0.642
1.272
0.498
0.491
0.445
0.463

  • ↵* P < 0.05 compared with incubations conducted without albumin.